Ultrasensitive Electrical DNA Identification with Bio-Bar-Code DNA and Nanoparticles in Nanogap Device

Tien-Li  Chang; Dar-Sun  Liou; Hung-Yi Lin; Fuh-Yu  Chang; Ping-Hei Chen

doi:10.6180/jase.2007.10.2.06

Ultrasensitive Electrical DNA Identification with Bio-Bar-Code DNA and Nanoparticles in Nanogap Device

Tien-Li Chang This email address is being protected from spambots. You need JavaScript enabled to view it.¹, Dar-Sun Liou² , Hung-Yi Lin¹ , Fuh-Yu Chang¹ and Ping-Hei Chen²

¹Mechanical and Systems Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan 310, R.O.C.
²Department of Mechanical Engineering, National Taiwan University, Taipei, Taiwan 106, R.O.C.

Received: February 26, 2007
Accepted: April 3, 2007
Publication Date: June 1, 2007

Download Citation: ||https://doi.org/10.6180/jase.2007.10.2.06

ABSTRACT

An electrical detection method for an ultrasensitive approach to identify DNA strands with self-assembled multilayer gold nanoparticles (AuNPs) in nanogap device is presented. This approach provides high selectivity with sensitivity that can be comparable to nanogap sensing on-chip system without the need of polymerase chain reaction (PCR) amplification. The concentration of target DNA is amplified at least 100 folds utilizing bio-bar-code DNA approach which is a pseudo-homogeneous system with two nanoparticle-based solutions including magnetic/gold nanoparticles (MNPs/AuNPs). This study indicates that lower concentration of the probes can be used very efficiently to bind target DNA, thereby reducing time required for DNA sensing. The nanogap devices for DNA identification are fabricated by electron-beam lithography (EBL). The concept for electrical detection of biological molecular interaction herein will be an excellent dynamic range and ideally set up for multiplexing in the future. Based on bio-bar-code DNA amplification (BCA) detection technique, the target DNA can be detected the concentration even lower than femto-molar range.

Keywords: : Electrical Detection, DNA Biochip, Bio-Bar-Code DNA, Magnetic Nanoparticles, Gold Nanoparticles, Nanogap, Self-Assembled Multilayer

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