Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract

Malik Ahmad  Al-Natour; Mohammad Faiyaz  Anwar; Shah Alam  Khan; Mohammad  Changez

doi:10.6180/jase.202510_28(10).0006

Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract

$Characterization of Ag NPs synthesized using aqueous OS leaf extract (a) UV-vis spectra of Ag NPs (spherical and nano-rods) and (b) thin film X-ray diffraction pattern of Ag spherical nanoparticles and nanorods$

Malik Ahmad Al-Natour¹, Mohammad Faiyaz Anwar², Shah Alam Khan³, and Mohammad Changez⁴This email address is being protected from spambots. You need JavaScript enabled to view it.

¹College of Health Sciences, University of Buraimi, Oman

²Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India

³Department of Pharmaceutical Chemistry, College of Pharmacy, National University of Science and Technology, Muscat, Oman

⁴Research, Innovation, and Entrepreneurship Unit, University of Buraimi, Oman

Received: March 7, 2024
Accepted: October 14, 2024
Publication Date: January 13, 2025

Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Download Citation: ||https://doi.org/10.6180/jase.202510_28(10).0006

Ocimumsanctum (OS) Linn, is a medicinal herb that has been used in traditional medicine science ancient time. It contains an array of complex and diverse biologically active phytochemical constituents that can act as a reducing and stabilizing agent for synthesizing metal nanoparticles (NPs). Herein, OS aqueous leaf extract was used to synthesize eco-friendly round and rod-shaped Ag NPs. The synthesized spherical and rod-shaped AgNPswereanalyzed by indirect methods by analysis of plasma band (UV-vis spectra) and thin films X-ray diffraction (XRD) peaks. Whereas, for direct methods, morphologies of synthesized Ag NPs were recoded using HR-TEM. AgNPsdemonstrated dose-dependent antibacterial efficacy against bacteria ( S. aureus). The MIC and MBCvalues of spherical Ag NPs against 106CFU/mL of S. aureus were found to be 2.74 and 3.80 µg/mL, respectively. However, MIC and MBC values of Ag nano-rod were observed at 7.20µ g/mL and 13µ g/mL, respectively. SEM micrographs indicate that morphologies of spherical shape and nano-rod Ag NPs treated S. aureus showed breakdown, merge, and elongated morphologies with the presence of Ag NPs (nano-rod) inside of the S. aureus.

Keywords: Green Chemistry, Ocimum sanctum, Staphylococcus aureus, Silver Nanoparticles, Transmission electron microscope, XRD

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